Carrier Proteins of Folic Acid Activity in Human Serum

Abstract

The natural folic acid activity (FAA) in the serum, on Sephadex® G-200 gel filtration, is divided into 3 fractions: the first in the band of largemolecular proteins; the second in that of small-molecular proteins; and the third outside the protein band. The second fraction, in the present study, was purified by hydroxyapatite chromatography, with the result that FAA emerged over a narrow area of fractions. In immunoelectrophoresis anddisc electrophoresis this area contained only albumin