Auxiliary‐Mediated Site‐Specific Peptide Ubiquitylation

Abstract

Lighting up ubiquitylation: A photolytically removable ligation auxiliary (Aux) was employed for the site-specific attachment of ubiquitin (Ub) to one of three lysine residues in a C-terminal peptide fragment of histone H2B (cH2B). Following photolysis, the ligation product was a viable substrate for the ubiquitin C-terminal hydrolase UCH-L3. Supporting information for this article is available on the WWW under http://www.wiley-vch.de/contents/jc_2002/2007/z605155_s.pdf or from the author. Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.