SODIUM PREGNANEDIOL GLUCURONIDE: THE SIGNIFICANCE OF ITS EXCRETION IN THE URINE¹¹

Abstract

THERE HAS been need for an accurate clinical test of ovarian function, especially one which yields information in regard to the presence and function of the corpus luteum. Attempts, however, to identify the active principle of the corpus luteum in the tissues and fluids of the human female, generally have been disappointing when various methods of bioassay were employed. Only small amounts of progestin were found by Adler and coworkers (1) and by Ehrhardt(2)in human placentas and in the urines of pregnant women. Clauberg and his associates (3)obtained a questionable progestational reaction with an extract prepared from approximately 50 gm. of human corpora lutea. Pratt and his group (4), however, were able to get positivetests in the immature rabbit with extracts equivalent to 60 gm. of corpora lutea. Loewe and Voss (5) found only one rabbit unit of biologically active progestin in 20 liters of urine from women during the last 5 days of the menstrual cycle.