Technical Requirements in the Determination of Absorption Spectra by the Ultraviolet Microscope

Abstract

To overcome the difficulties and limitations of earlier methods for obtaining absorption spectra of detailed microscopic structures, it has been found necessary to impose more rigid demands upon the monochromator and to introduce improvements in densitometry. The optical properties of the microscope and the spectral properties of the source together determine the requirements which must be made upon the monochromator. These requirements are analyzed and a convenient method of quantitative densitometry is described together with the results of a simple test of this method.