Journal of the American Chemical Society · 2002 · 160 citations · 4 references
Life SciencesBiotechnologyMolecular BiologyMolecular BiotechnologyPolymer ChemistrySynthetic BiologyDna StructureNucleic Acid EngineeringNucleic Acid SynthesisOligonucleotide ChemistryPolymerization ProcessesStep-growth PolymerizationAmination ReactionsBase Pair MismatchesChemical Modification Of OligonucleotidesDna-templated SynthesisOligonucleotide SynthesisStep-growth KineticsStep-growth Polymerization KineticsSynthetic Polymers
Native DNA oligomers are shown to be stereoselective catalysts for the polymerization of 5'-amino-3'-acetaldehyde-modified thymidine/adenosine nucleosides through reductive amination. The reaction follows step-growth kinetics to read the encoded sequence and chain-length information in the antiparallel direction. Single mismatches in the template are selected against at a level of >100:1. A method is therefore established to translate biopolymer-encoded information stereoselectively into sequence- and chain-length specific synthetic polymers.
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