High-throughput enzyme evolution in Saccharomyces cerevisiae using a synthetic RNA switch

Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method

R. Daniel Gietz, Robin A. Woods · Methods in enzymology on CD-ROM/Methods in enzymology · 2002 · 3K citations

Life Sciences, Biotechnology, Molecular Biology +6

Production of the antimalarial drug precursor artemisinic acid in engineered yeast

Dae‐Kyun Ro, Eric M. Paradise, Mario Ouellet et al. · Nature · 2006 · 2.8K citations

Life Sciences, Biological Engineering, Biotechnology +12

Isoprenoid Pathway Optimization for Taxol Precursor Overproduction in <i>Escherichia coli</i>

Parayil Kumaran Ajikumar, Wenhai Xiao, Keith E. J. Tyo et al. · Science · 2010 · 1.6K citations

Engineering, Biochemistry, Biological Engineering +17

Programming cells by multiplex genome engineering and accelerated evolution

Harris H. Wang, Farren J. Isaacs, Peter A. Carr et al. · Nature · 2009 · 1.6K citations

Life Sciences, Biotechnology, Genetics +12

Protein stability promotes evolvability

Jesse D. Bloom, Sy T. Labthavikul, Christopher R. Otey et al. · Proceedings of the National Academy of Sciences · 2006 · 1.3K citations · Full text

Life Sciences, Biochemistry, Evolutionary Biology +17