Nuclear and cytoplasmic mRNA quantification by SYBR green based real-time RT-PCR

Accurate normalization of real-time quantitative RT-PCR data by geometric averaging of multiple internal control genes

Jo Vandesompele, Katleen De Preter, Filip Pattyn et al. · Genome biology · 2002 · 20.1K citations · Full text

Life Sciences, Molecular Biology, Molecular Genetics +14

Real time quantitative PCR.

Chris Heid, Junko Stevens, Kenneth J. Livak et al. · Genome Research · 1996 · 6.2K citations · Full text

Life Sciences, Biotechnology, Molecular Biology +13

Determination of stable housekeeping genes, differentially regulated target genes and sample integrity: BestKeeper – Excel-based tool using pair-wise correlations

Michael W. Pfaffl, Aleš Tichopád, Christian Prgomet et al. · Biotechnology Letters · 2004 · 5.1K citations

Life Sciences, Genetics, Molecular Biology +12

Kinetic PCR Analysis: Real-time Monitoring of DNA Amplification Reactions

Russell Higuchi, Carita Fockler, Gavin Dollinger et al. · Nature Biotechnology · 1993 · 2.2K citations

Life Sciences, Biotechnology, Molecular Biology +16

Validation of housekeeping genes for normalizing RNA expression in real-time PCR

Keertan Dheda, Jim F. Huggett, Stephen A. Bustin et al. · BioTechniques · 2004 · 1K citations · Full text

Life Sciences, Microbiology, Molecular Biology +10