Integrated Biocatalytic Synthesis on Gram Scale: The Highly Enantioselective Preparation of Chiral Oxiranes with Styrene Monooxygenase

Abstract

Enantiopure oxiranes have been prepared on a gram scale by recombinant E. coli JM101 (pSPZ10) containing styrene monooxygenase in an emulsion process including facile and efficient downstream processing. This biocatalyst was compared to the chemical catalysts introduced by Jacobsen et al. with respect to its performance for epoxidations of vinyl groups.