The Development of a High Performance Liquid Chromatograph with a Sensitive On-stream Radioactivity Monitor for the Analysis of³H- and¹⁴C-labelled Gibberellins

Abstract

The development of a high performance liquid chromatograph for the separation of gibberellins is described. The system combines high efficiency, peak capacity, and sample capacity with rapid speed of analysis. In addition, the constructional details of a sensitive on-stream radioactivity monitor are outlined. The overall versatility of the chromatograph is demonstrated by the separation of a range of 3H- and 14C-labelled Gibberellins and gibberellin precursors. The system also has considerable potential for the analysis of abscisic acid and acidic and neutral indoles.