Rapid Communications in Mass Spectrometry · 2004 · 104 citations · 31 references
GlycobiologyBiological Mass SpectrometryPolysaccharideCarbohydrate-protein InteractionSpectrochemical AnalysisFood ChemistryTandem Mass SpectrometryAnalytical ChemistryFood SciencesNew Fragmentation MechanismsChromatographyGlycosylationLaser Desorption/ionizationBiochemistryLinkage PositionsBiomolecular EngineeringIon MobilityNatural SciencesMass SpectrometryProtein Mass SpectrometryNative Mass SpectrometryMedicineHuman MilkMolecular Fragmentation
Abstract The spectra recorded by matrix‐assisted laser desorption/ionization time‐of‐flight/time‐of‐flight tandem mass spectrometry (MALDI‐TOF/TOF‐MS/MS) of complex carbohydrates from human milk are presented. Besides ions originating from glycosidic cleavages and from sugar ring fragmentations, these spectra show intense peaks that may be assigned to ions produced by three new fragmentation pathways involving a six‐atom rearrangement. These ions, together with the A fragments from sugar ring fragmentations, open the possibility of obtaining a complete mapping of the linkage positions present in the carbohydrates investigated by MALDI‐TOF/TOF. Copyright © 2004 John Wiley & Sons, Ltd.
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Katalin F. Medzihradszky, J. M. Campbell, Michael A. Baldwin et al. · Analytical Chemistry · 1999 · 526 citations
Biochemistry, Peptide Collision-induced Dissociation, High Sensitivity +15