Journal of the American Chemical Society · 2006 · 326 citations · 7 references
Life SciencesBiochemistryBiophysicsProtein BiochemistrySpectroscopyStructural BiologyMacromolecular Structure DeterminationMagnetic Resonance SpectroscopyProtein DynamicsProtein StructureSpectroscopy Of BiomoleculesMultidimensional Nmr SpectroscopyMultidimensional Nuclear Magnetic Resonance SpectroscopyNuclear Magnetic Resonance Structure DeterminationProtein Structure AnalysisProtein Structure DeterminationProtein Turnover KineticsProtein Unfolding KineticsThree-dimensional Nmr Spectroscopy
We demonstrate for different protein samples that three-dimensional HNCO and HNCA correlation spectra may be recorded in a few minutes acquisition time using the band-selective excitation short-transient sequences presented here. This opens new perspectives for the NMR structural investigation of unstable protein samples and real-time site-resolved studies of protein kinetics.
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