Extraction of Human Genomic DNA from Dried Blood Spots and Hair Roots

Abstract

In most studies about molecular genetics, molecular diagnostics, DNA extraction is considered as the very first step that will give a significant effective. The material used for DNA extraction is vary from fresh blood to dried blood, tissue, oral mucosa and hair root. The dried blood and hair root are the two materials that easy to collect and can store for long term, which leads to more advantages for many time extractions to have fresh DNA and enough amount DNA for further studies. This study introduces an efficient phenol method with 2 steps of lyses which allows high yield of DNA concentration from dried blood and hair root. Comparing to the commercial QIAamp DNA Mini Kit which applied for dried blood sample, DNA concentration extracted from this method was higher, the quality of DNA was equal and the cost is lower. Comparing to salting out method, a cheap and safe method, which an applied for hair root, the two-step lysis phenolic method also give higher efficiency. This method could be the useful tool which may apply for further molecular analysis studies using long storing time materials like dried blood spots and hair root samples.