Effects of Solvent Upon Absorption Spectra of Chlorophylls A and B; Their Ultraviolet Absorption Spectra in Ether Solution

Abstract

1. Solutions of chlorophyll a were prepared in thirteen solvents and of chlorophyll b in five solvents by direct elution from sucrose adsorption columns. 2. Absorption spectra for each solvent are reported as measured spectrophoto-electrically in the visible region. 3. Spectra for the ultraviolet region to 2650 A were determined for solutions in ethyl ether. 4. A preparation of dried chlorophyll a was made which retained excellent spectroscopic properties. 5. The application of Kundt's rule to chlorophyll solutions is discussed, as well as certain relations of spectroscopic properties and solvents to analytical applications.