Proceedings of the National Academy of Sciences · 2003 · 74 citations · 22 references
Life SciencesGeneticsMicrobiologyDna RepairMicrobial GeneticsRecombinationHomologous RecombinationMicrobial RecombinationRecombination EnzymesRecombination IntermediatesRecombination ProteinsBacterial Recombination MechanismsBacterial Recombination ProteinsHomologous Recombination AssemblyHomologous Recombination IntermediatesHomologous Recombination RepairSingle-stranded Dna Structure
Bacillus subtilis RecU protein is involved in homologous recombination, DNA repair, and chromosome segregation. Purified RecU binds preferentially to three- and four-strand junctions when compared to single-stranded DNA (ssDNA) and double-stranded DNA (dsDNA) ( approximately 10- and approximately 40-fold lower efficiency, respectively). RecU cleaves mobile four-way junctions but fails to cleave a linear dsDNA with a putative cognate site, a finding consistent with a similar genetic defect observed for genes classified within the epsilon epistatic group (namely ruvA, recD, and recU). In the presence of Mg(2+), RecU also anneals a circular ssDNA and a homologous linear dsDNA with a ssDNA tail and a linear ssDNA and a homologous supercoiled dsDNA substrate. These results suggest that RecU, which cleaves recombination intermediates with high specificity, might also help in their assembly.
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The complete genome sequence of the Gram-positive bacterium Bacillus subtilis
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The importance of repairing stalled replication forks
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