Proceedings of the National Academy of Sciences · 2011 · 481 citations · 21 references
Life SciencesBiophysicsCell BiologyApoptosisCell BiophysicsMolecular BiophysicsApoptosis BiophysicsCytochrome C ReleaseMitochondrial BiochemistryMitochondrial MetabolismMitochondrial PhysiologyRaman SpectroscopyLabel-free MicroscopyMitochondrial Cytochrome C MobilizationMitochondrial Dynamics In ApoptosisMitochondrial Redox Regulation Of ApoptosisMitochondrial Redox StateRaman ImagingRaman MicroscopyRaman Microscopy Of Cells
We performed label-free observation of molecular dynamics in apoptotic cells by Raman microscopy. Dynamic changes in cytochrome c distribution at the Raman band of 750 cm(-1) were observed after adding an apoptosis inducer to the cells. The comparison of mitochondria fluorescence images and Raman images of cytochrome c confirmed that changes in cytochrome c distribution can be distinguished as release of cytochrome c from mitochondria. Our observation also revealed that the redox state of cytochrome c was maintained during the release from the mitochondria. Monitoring mitochondrial membrane potential with JC-1 dye confirmed that the observed cytochrome c release was associated with apoptosis.
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