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A possible function of cyclic AMP in the induction of polyphenol oxidase preceding root formation in cultured carrot-root callus
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1977
Year
BotanyLipid PeroxidationRoot FormationCulture MediumRedox BiologyPolyphenolicsOxidative StressBiosynthesisPolyphenol OxidaseOther Adenosine MonophosphatesBiochemistryCyclic AmpReactive Oxygen SpecieMetabolomicsCell BiologyBiologyNatural SciencesBiotechnologyPlant Cell CultureRoot MorphologyMetabolismMedicinePlant Physiology
The stimulative effect on polyphenol oxidase activity preceding root formation in cultured calluses is specific to cAMP since other adenosine monophosphates failed to exert a cAMP-like effect for increasing the enzymatic activity. Addition of 5-fluorouracil (Fu) to the culture medium in the presence of IAA (10−3 mg/liter) resulted in an alteration in the thermolability of polyphenol oxidase and, this Fu action was promoted in the presence of cAMP. In addition, actinomycin D counteracted the cAMP-induced stimulation. These results indicate that cAMP is able to stimulate transcription of the structural gene for polyphenol oxidase which is related to root formation in cultured tissues.