Surface vs. solution hybridization: effects of salt, temperature, and probe type

Wanqiong Qiao, Hao-Chun Chiang, Hui Xie, Rastislav Levicky

Chemical Communications · 2015 · 35 citations · 28 references

Concepts

Abstract

Hybridization thermodynamics on solid supports are compared with those in solution for two types of hybridization probe, DNA and uncharged morpholino oligonucleotides of identical sequences. Trends in hybridization affinity are discussed with respect to ionic strength, temperature, and surface behavior.

References

28