The Journal of Biochemistry · 1988 · 95 citations · 0 references
Life SciencesMarine BiologyMicrobiologyMarine MicrobiologyMicrobial MetabolismMicrobial PhysiologyEicosanoid BiosynthesisFatty Acid MetabolismMarine Microbial MetabolismMarine Microbial PhysiologyMicrobial GrowthMicrobial Metabolite ProductionCultivation Of Marine MicroorganismsDoubling Time AnalysisEicosapentaenoic AcidFatty AcidsMarine Fatty AcidsMarine Microbial CultivationPopulation Doubling TimeRod-shaped Morphology
About 5,000 strains of marine microorganisms were screened for eicosapentaenoic acid (EPA)-producing ability, which was detected in 88 of them. All of the latter were found to be obligate aerobic, Gram-negative, motile, short rod-shaped bacteria. One strain, designated as SCRC-8132, showed a doubling time of 30 min at 25 degrees C and produced 20 mg/liter (4 mg/g dry cells) when cultured in a P-Y-M-Glucose medium for 18 h. The EPA to total fatty acids ratio was 24%. The strain produced 26 mg EPA/liter (15 mg/g dry cells) when cultured at 4 degrees C for 5 days, the EPA ratio being increased to 40%.