Oncogenic purine N-oxide derivatives as substrates for sulfotransferase.

Abstract

Summary The susceptibility of a series of purine N-oxide derivatives to rat liver sulfotransferase has been measured by a new assay. With 3′-phosphoadenylyl sulfate-35S as the donor, the 35S-labeled sulfate liberated by the rapid reaction of the resulting sulfate ester is a measure of the acceptor activity of the substrate. The susceptibility of purine N-oxide derivatives to sulfotransferase is not proportional to their oncogenicity. The assay was also used to detect purine N-oxide sulfotransferase activity in a type of fibroblast grown in culture.