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Determination of Lipoproteins by Paper Electrophoresis
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1958
Year
Lipid AnalysisEngineeringBioanalysisAnalytical ChemistryClinical ChemistryIsotachophoresisChromatographyExcess DyeCapillary ElectrophoresisBiochemistryChemical PathologyBiomedical AnalysisLipid SciencePaper ElectrophoresisReproducible TechnicBiomolecular EngineeringSudan Black BBiotechnologyLipoprotein MetabolismLipid ChemistryMedicine
Abstract A reproducible technic is presented for the staining of lipoproteins on paper. This method gives a greater total uptake of dye than previously described procedures, especially by the α-lipoproteins. The staining is accomplished with a solution of Sudan Black B in a mixture of methyl and isopropyl alcohols and the excess dye is removed by wash solution of 30% isopropanol.