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A Histological Study of Development of Adventive Embryos in Organ Cultures of Phoenix dactylifera L.
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1980
Year
BotanyAnatomyReproductive BiologyAdventive EmbryosEmbryologyPlant DevelopmentEmbryo CulturePlant CytologyHealth SciencesCallus CulturesBlastemaPhoenix Dactylifera LHistological StudyMorphologyMorphogenesisEmbryonic DevelopmentOrganogenesisCell BiologyBiologyDevelopmental BiologyPlant Cell CultureOntogenyGlobular ProembryosTissue CultureMedicinePlant Physiology
Callus cultures from axillary buds of 2–4-year-old offshoots of Phoenix dactyl'fera L have yielded plantlets adventitiously Adventive embryos originated from meristematic cytoplasm-rich cells located in the epidermal and adjacent sub-epidermal portions of the periphery of callus on a basal medium supplemented with 2, 4-D and isopentenyladenine The formation of adventive embryos is described Morpho-genetically competent cells proliferate into globular proembryos which differentiate into bipolar structures. Transfer to a medium devoid of hormones enhances embryo development and promotes plantlet growth Comparative studies were made between zygotic and adventive embryos in various stages of development