HPTLC FINGER PRINT ANALYSIS AND PHYTOCHEMICAL INVESTIGATION OF MORINDA TINCTORIA ROXB LEAF EXTRACTS BY HPLC AND GS MS

Abstract

Objective: The present study was conducted to investigate the qualitative and quantitative screening of phytochemicals, and characterization by HPTLC finger print analysis, HPLC and GC MS substantiation for the presence of scopoletin and rutin in methanolic leaf extracted of Morinda tinctoria Roxb. (MEMT). Methods: The Qualitative and Quantitative Phytochemical analyses were carried out as per the standard protocols. The MEMT characterization was performed by High-Performance Thin Layer Chromatography (HPTLC), High-Performance Liquid Chromatography (HPLC) and Gas Chromatography-Mass spectrometry (GC-MS). Results: The preliminary phytochemical screening of methanolic extracts revealed the presence of alkaloids, flavonoids, phenolics, terpenoids, saponins, tannins, phlobatanin, quinones, coumarins, cardiac glycosides and proteins. The quantitative phytochemicalscreenings in terms of total alkaloids, total flavonoids, total phenolic, total proteins and DPPH scavenging activity of MEMT were found to be 13 ± 0.034 mg/g, 0.06±0.003 mg/g, 0.08 ±0.012 mg/g, 0.29 ± 0.071 mg/g and 62.12 ± 0.250 %. From the HPTLC results, the R f values of scopoletin and rutin were calculated as 0.55 and 0.24. Scopoletin and Rutin content of MEMT was 1.58 and 1.88 % w/w of air dried to extract by HPLC. GC-MS analysis of the MEMT showed the presence of compounds like scopolamine, a secondary metabolite and Malvidin-3, 5-diglucoside, an anthocyanin and a glycoside, as main constituents. Conclusion: From our results, we found that the proposed HPLC method is fast, discriminating, requires trouble-free sample preparation procedure, and regarded as a good method for quantification in different plant extracts and for regular quality control of herbal formulations.