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Serum Lipase Determination with an Olive Oil Substrate Using a Three-Hour Incubation Period
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1962
Year
Three-hour Incubation PeriodLipid AnalysisEngineeringCherry-crandall ProcedureFood ChemistrySerum Lipase DeterminationBioanalysisBiochemical EngineeringAnalytical ChemistryClinical ChemistryOlive OilChromatographyBiochemistryLipid ResourceSerum LipaseBiomolecular EngineeringLipid PreparationBiotechnologyMedicine
Abstract The effect of various emulsifying agents, pH, buffers, and accelerators on the Cherry-Crandall procedure for determining serum lipase was studied. A method is proposed which uses a 3-hour incubation period. Olive oil emulsified with 7% acacia is the substrate. This is buffered with tris buffer, pH 8.0; magnesium acetate and EDTA are added as accelerators. Other conditions which result in an increased rate of hydrolysis are discussed.