A Simple Method for Cutting Sections in the 0·5 to 1 μ Range, and for Sections of Chitin

Abstract

ABSTRACT Tissues fixed and stained with osmium/ethyl gallate often require sectioning at 0·5 to 1 µ. In the method described they are double-embedded in agar and ‘ester wax’ (Steedman), cut on a film of water on the coverslip, and mounted in gum containing ethyl gallate. Prepared safety razor blades are recommended. This method is useful also for cutting serial sections of insect cuticle.