FEMS Microbiology Letters · 2007 · 15 citations · 38 references
Life SciencesGeneticsMicrobiologyBacteriologyMicrobial GeneticsMicrobial PathogenesisBacterial Gene RegulationBacterial PathogenesisBacteriophage GeneticsGene Regulation In MicrobesMicrobial TranscriptomicsPhage GeneticsMitomycin C TreatmentPhage CharacterizationPhage GenomicsProphage BiologyPseudomonas FluorescensPseudomonas SpeciesReca ProteinReca-mediated Recombination
DNA microarray technology was used to survey changes in gene expression in Pseudomonas fluorescens after mitomycin C treatment. As expected, genes associated with the SOS response were upregulated, such as those encoding the recombination protein RecA, DNA repair protein RecN, excinuclease ABC subunit A UvrA, and the LexA repressor protein. Interestingly, expression of 33 clustered bacteriophage-like genes was upregulated, suggesting that mitomycin C (MMC) may induce a prophage resident in the P. fluorescens genome. However, no phage particles were detected in P. fluorescens strain DC206 that had been treated with MMC using transmission electron microscopy. The same preparation failed to produce phage plaques on lawns of any of 10 different Pseudomonas strains tested, indicating that the 33 bacteriophage-like gene cluster represents a defective prophage.
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Complete genome sequence of the plant commensal Pseudomonas fluorescens Pf-5
Ian T. Paulsen, Caroline M. Press, Jacques Ravel et al. · Nature Biotechnology · 2005 · 658 citations · Full text
Genome-wide expression profiling in Escherichia coli K-12
Craig S. Richmond · Nucleic Acids Research · 1999 · 578 citations · Full text