Antimicrobial Agents and Chemotherapy · 1996 · 504 citations · 22 references
Life SciencesInfectious DiseasesMicrobiologyAntimicrobial ResistanceBacteriologyMedical MicrobiologyAntibiotic ResistanceAntibiotic Resistance EvolutionAntibiotic Resistance GeneticsAntibiotic Resistance MechanismsPeptic Ulcer DiseaseResistance GenesClarithromycin ResistanceHelicobacter Pylori InfectionMutationMutation-driven ResistanceMutational Pathways Of ResistancePoint Mutation AnalysisTransition MutationsTreatment-resistant Peptic Ulcer Disease
Twelve clarithromycin-resistant Helicobacter pylori isolates (100% of resistant isolates examined) from seven different patients each contained an A-->G transition mutation within a conserved loop of 23S rRNA. A-->G transition mutations at positions cognate with Escherichia coli 23S rRNA positions 2058 and 2059 were identified. Clarithromycin-susceptible H. pylori isolates from 14 different patients displayed no polymorphisms in a conserved loop within domain V of 23S rRNA. The study is the first to report mutations in H. pylori associated with resistance to an antimicrobial agent used in established peptic ulcer treatment regimens.
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