Serum Cholesterol Assay Using a Stable Liebermann-Burchard Reagent

Abstract

Abstract A single stable Liebermann-Burchard reagent was prepared and studied for the rapid and accurate determination of total cholesterol in serum. The preparation, stability, and developed spectral character of the proposed reagent were assessed. Reproducibility, recovery, and interference studies on pure standard and patient sera were performed using the Abell-Kendall method as reference. The influence of water, protein, bilirubin, hemoglobin, and other serum constituents was evaluated.