PubMed · 1999 · 68 citations · 0 references
Veterinary MedicineInfectious DiseasesVeterinary MicrobiologyDiagnostic MicrobiologyFood SafetyFoodborne Disease EpidemiologyBacterial ToxinsPathogen DetectionToxin ProductionVeterinary Diagnostic MicrobiologyBacterial Toxin AssaysBacterial Toxin EpidemiologyEscherichia ColiShiga Toxin-producing Escherichia Coli DetectionShiga ToxinsToxin Detection AssaysToxin-producing Bacteria
From October to December 1998, single faecal samples from 67 healthy cattle in a semi-urban community near Calcutta were examined for Shiga toxin producing Esch. coli (STEC) using a multiplex PCR primary screen followed by plating on sorbitol MacConkey agar. STEC was isolated from the faeces of 7 (10.5%) animals. The eight strains isolated belonged to eight serotypes viz, O146:H1, O149:HNT, ONT:H34, ONT:H19, O88:HN, ONT:H2, O82:H8 and O28ac:H21. Bead enzyme linked immunosorbent assay showed that three strains produced Shiga toxin 1, one produced Shiga toxin 2 and four produced both.