The purification of alkaline phosphatases of animal tissues

Abstract

C,5H15O0NCI requires C, 65-8; H, 4-4; N, 541; Cl, 13-0%.) Cyclization of the chloroacetyl derivative was effected by dissolving it (5-36 g.) in hot ethanol (50 ml.) and diluting with water (140 ml.). When the solution became clear again, NaOH (115 g.) in water (10 ml.) was added, and a yellow colour developed owing to partial hydrolysis. The solution was refluxed for 30 min. and acidified with HCI while still hot. On cooling, colourless, chlorine-free, prismatic needles separated and were collected (4.3 g., 72% overall yield, m.p. 240-242). Crystallization from ethanol gave synthetic viridicatin, m.p. 264-265', not depressed on admixture with natural viridicatin from P. cydopium.