Cell-Free Synthesis of the Rice Glutelin Precursor

Abstract

Polyadenylated RNA was directly isolated from developing rice (Oryza sativa. L) seeds. The major translation product of the isolated polyadenylated RNA in a rabbit reticulocyte cell-free system was about 2 kDa larger than the in vivo labeled 57 kDa glutelin precursor, and was immunoprecipitated by antiserum against the glutelinprolamin fraction of mature seeds.