Processing of the carbohydrate units of thyroglobulin.

Abstract

Conditions of incubation and collection of immunoprecipitate are described under "Experimental Procedures."Anti-calf thyroglobulin antibodies were prepared from immunized rabbits (see "Experimental Procedures").-, no thyroglobulin antibody was added.Obtained from nonimmunized rabbits and added at an equivalent 'Goat anti-rabbit immunoglobulin antiserum (Miles Biochemicals).protein concentration.convenience, the oligosaccharides were initially designated a to e. Oligosaccharide a migrated to the position of the GlcaMan9GlcNAc standard while the mobility of oligosaccharide b was identical with that of Glc2Man9GlcNAc.In order to