Concepedia

In vitro gene expression within membrane-free coacervate protocells

T.‐Y. Dora Tang, Dirk van Swaay, Andrew J. deMello, J. L. Ross Anderson, Stephen Mann

Chemical Communications · 2015 · 194 citations · 24 references

DOIFull text

Open access

Concepts

Abstract

Cell-free gene expression of a fluorescent protein (mCherry) is demonstrated within the molecularly crowded matrix of a polysaccharide/polypeptide coacervate.

References

24

Cell-free translation reconstituted with purified components

Yoshihiro Shimizu, Akio Inoue, Yukihide Tomari et al. · Nature Biotechnology · 2001 · 2K citations

, , +9

A vesicle bioreactor as a step toward an artificial cell assembly

Vincent Noireaux, Albert Libchaber · Proceedings of the National Academy of Sciences · 2004 · 1.2K citations · Full text

, , +17

Man-made cell-like compartments for molecular evolution

Dan S. Tawfik, Andrew D. Griffiths · Nature Biotechnology · 1998 · 943 citations

, , +11

Peptide–nucleotide microdroplets as a step towards a membrane-free protocell model

Shogo Koga, David Williams, Adam W. Perriman et al. · Nature Chemistry · 2011 · 674 citations

, , +13

Interfacial assembly of protein–polymer nano-conjugates into stimulus-responsive biomimetic protocells

Xin Huang, Mei Li, David C. Green et al. · Nature Communications · 2013 · 532 citations · Full text

, , +16