Pilin expression in Neisseria gonorrhoeae is under both positive and negative transcriptional control.

DNA sequencing with chain-terminating inhibitors

Frederick Sanger, S. Nicklen, Alan Coulson · Proceedings of the National Academy of Sciences · 1977 · 69.1K citations · Full text

Dna, Engineering, Dna Analysis +20

Improved M13 phage cloning vectors and host strains: nucleotide sequences of the M13mpl8 and pUC19 vectors

Celeste Yanisch-Perron, Jeffrey Vieira, Joachim Messing · Gene · 1985 · 15.1K citations

Genetics, Puc19 Vectors, Bacteriophage +9

Studies on transformation of Escherichia coli with plasmids

Douglas Hanahan · Journal of Molecular Biology · 1983 · 11.2K citations

Bacteriology, Biotechnology, Escherichia Coli +4

Analysis of the accuracy and implications of simple methods for predicting the secondary structure of globular proteins

J. Garnier, David J. Osguthorpe, Barry Robson · Journal of Molecular Biology · 1978 · 4.6K citations

Structural Bioinformatics, Biomolecular Structure Prediction, Protein Folding +13

Rapid and Sensitive Protein Similarity Searches

David J. Lipman, William R. Pearson · Science · 1985 · 4.1K citations