Biotechnology and Applied Biochemistry · 1989 · 27 citations · 0 references
Life SciencesBiochemistryBiotechnologyBioseparationsEnzymologyProtein BiochemistryEnzyme ImmobilizationEnzyme IsolationEnzyme PurificationEnzyme RecoveryImmobilized EnzymesProtein PurificationAdsorption ImmobilizationAdsorption-based Enzyme RecoveryBuffer Composition EffectsChromatographic Enzyme PurificationEnzyme Affinity PurificationEnzyme FractionationEnzymesProtein Purification By Adsorption
Two enzymes, glycogen phosphorylase and lactate dehydrogenase, were purified simultaneously in a single step. Ferric ions immobilized on a chelating gel were used as the adsorbent. Adsorption and desorption steps were accomplished by changes in buffer composition. The recoveries were better than 80% and the capacities were about 5 mg of protein per milliliter of adsorbent. The procedure worked well both on a small and on a preparative scale. The homogeneity of the purified enzymes was checked by FPLC.