The Journal of Biochemistry · 1985 · 22 citations · 0 references
Life SciencesBiochemistryMicrobiologyEnzymologyGlycobiologyStructural BiologyCell Wall BiosynthesisGlycan AnalysisGlycan BiosynthesisGlycan DegradationGlycan StructurePolysaccharide StructureAlkali TreatmentBeta-glucanase TreatmentCell Wall Polysaccharide HydrolysisGlycan Structural AnalysisGlycan Structure AnalysisGlycan TopologyThree-dimensional Glycan StructureYeast Cell Wall Biology
A mutant of Saccharomyces cerevisiae defective in the cell wall beta-glucan structure was obtained. The mutant cells are extremely sensitive to (beta 1-3)-glucanase digestion and mild alkali treatment. Structural analysis revealed that the alkali-insoluble, skeletal glucan from wild type cells contains two components, a (beta 1-3) linked glucan with a laminated structure, and a highly branched glucan containing predominantly (beta 1-6) linkages. The mutant cells lack the latter component.