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Effect of interleukin-1 on gamete interaction and mouse embryo development.
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1990
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FertilityLymphocyte DevelopmentImmunologyGynecologyImmunologic MechanismReproductive BiologyImmune SystemCulture MediumFertilisationEmbryologyInflammationEmbryo CultureEarly StagesPublic HealthImmune MediatorCell SignalingGamete InteractionInfertilityCell DivisionEndocrinologyCell BiologyHuman ReproductionCytokineDevelopmental BiologyImmune Cell DevelopmentDevelopmental ImmunologyMedicineSperm VelocityCell Development
Early stages of endometriosis have been shown to be associated with infertility. The pathophysiology of this relationship is unclear. To determine if interleukin-1 (IL-1), a peritoneal macrophage product, has any effect on gamete interaction and early embryo development, human recombinant IL-1 was added to the coincubation of gametes in the sperm penetration assay (SPA), human zona pellucida assay (ZPPA) and culture medium (Ham's F-10) used for processing semen samples with the layering method, with analysis of velocity and motility after 24 hours. IL-1 was also added to mouse embryos (two cells) cultured for 72 hours. The results showed that IL-1 caused impairment of SPA and ZPPA when compared to control medium, without significant alterations in sperm velocity and motility. Also, IL-1 demonstrated significant inhibition of mouse embryo development. These results help explain subfertility associated with early stages of endometriosis.