Electrophoretic comparison of myosins from masticatory muscles and selected limb muscles in the dog.

Abstract

Myosins from canine limb muscles (triceps brachii [medial and long heads], anconeus, and extensor carpi radialis) were compared biochemically with myosins from canine masticatory muscles (temporalis and masseter). Compared with the limb muscles, the temporalis and masseter muscles had: a unique myosin isoform pattern as determined by nondenaturing pyrophosphate gel electrophoresis; unique light chains as determined by 1-dimensional sodium dodecyl sulfate polyacrylamide gel electrophoresis, 2-dimensional gel electrophoresis, and peptide mapping; and a unique heavy chain as determined by peptide mapping.