Biochemical characterization of U2 snRNP auxiliary factor: an essential pre-mRNA splicing factor with a novel intranuclear distribution.

Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei

John David Dignam, Russell M. Lebovitz, R G Roeder · Nucleic Acids Research · 1983 · 11.6K citations · Full text

Engineering, Rna Polymerase Ii, Isolated Mammalian Nuclei +19

Rapid detection of octamer binding proteins with ‘mini extracts’, prepared from a small number of cells

Edgar Schreiber, Patrick Matthias, Michael M. Müller et al. · Nucleic Acids Research · 1989 · 4.2K citations · Full text

‘ Mini Extracts, Octamer Binding Proteins, Molecular Biology +17

A simple method of reducing the fading of immunofluorescence during microscopy

G.D. Johnson, Gloria M. de C. Nogueira Araujo · Journal of Immunological Methods · 1981 · 1.9K citations

Fluorescence Microscopy, Immunocytochemical Technique, Engineering +9

Splicing of Messenger RNA Precursors

Phillip A. Sharp · Science · 1987 · 921 citations

Genetics, Rna Splicing, Molecular Biology +16

Factor required for mammalian spliceosome assembly is localized to discrete regions in the nucleus

Xiang‐Dong Fu, Tom Maniatis · Nature · 1990 · 732 citations

Chromatin, Mammalian Spliceosome Assembly, Chromatin Structure +10