SPECTROPHOTOMETRIC DETERMINATION OF BLOOD OXYGEN

Abstract

Gasometric methods for estimating blood oxygen are time-consuming. This consideration often limits the number of observations which can be made in the course of experiments requiring oxygen determinations. In many cases it is an arteriovenous difference which is particularly desired, as in the measurement of cardiac output by the Fick principle, or the estimation of oxygen consumption by human liver, kidney, or brain. The present paper describes a rapid, simple spectrophotometric method for determination of arteriovenous oxygen differences with an