3-Carbanionic substrate analogues bind very tightly to fumarase and aspartase.

Abstract

The abbreviations and notations used are: Ia, Ib, and IC, tee conjugate 3-C acids of the nitroalkanes defined in Equation 2; IZ, Ib, and IC, the conjugate 3-C bases (3-carbanions) of Ia, Ib, and IC; Hdc, HIE, IcH-, and IC*-, the microscopic ionization states of IC defmed in Equation 8Ia,eq, Ib,eq and Ic,eq, solutions of Ia, Ib, and IC which are protonically equilibrated at 3-C.