Purification and partial characterization of a murine melanoma-associated antigen.

Abstract

A murine B16 melanoma-associated antigen was detected and partially purified from spent culture medium and intracellular material.The antigen was also purified and partially characterized from the detergent extract of cells cultured in the presence of [3H]glucosamine done or together with ['4C]leucine.During purification, the melanoma-associated antigen activity was monitored by a double antibody antigen binding assay (Bystryn,