Transcriptional regulation of the ovalbumin and conalbumin genes by steroid hormones in chick oviduct.

TLDR

The authors quantified ovalbumin and conalbumin transcription in isolated chick oviduct nuclei by measuring [32P]RNA synthesis hybridized to recombinant DNA, pulse‑labeling with [3H]uridine, and determining absolute rates from UTP pool activity. Estrogen or progesterone rapidly stimulated conalbumin transcription 2–3‑fold within 30 min and induced ovalbumin transcription at least 20‑fold over 12 h, though peak rates after 12 h were only 10–25 % of those seen after several days, matching low nuclear receptor levels and mirroring in‑vitro explant responses.

Abstract

Relative rates of ovalbumin and conalbumin mRNA transcription were measured in isolated oviduct nuclei by allowing endogenous RNA polymerases to synthesize [32P]RNA that was then hybridized to immobilized recombinant DNA containing the respective gene sequences. Administration of either estrogen or progesterone to withdrawn birds increased the rate of conalbumin mRNA (mRNA,,,) transcription 2- to 3-fold within 30 min. The rate of ovalbumin mRNA (mRNA,,) transcription was undetectable before hormone administration and increased at least 20-fold during the first 12 h. The maximum rates of transcription achieved after 12 h of hormonal stimulation are only 10 to 25% of those observed after several days of hormone treatment; these transcription rates are consistent with the low levels of nuclear estrogen or progesterone receptors measured during the first 12 h of induction. ), transcription was undetectable before hormone administrati... The induction of mRNA, and mRNAcon in oviduct explant cultures was quantitatively comparable to that observed in vivo. Relative rates of transcription were also measured in this system by pulse-labeling with [3H]uridine. In addition, absolute rates of transcription were determined by measuring the specific activity of the UTP pool during the labeling period. The accumulation of mRNA., sequences was consistent with the absolute rate of transcription measurements, indicating that this mRNA has a long