The Accumulation of Glucose 6-Phosphate from Glucose and Its Effect in an Escherichia coli Mutant Lacking Phosphoglucose Isomerase and Glucose 6-Phosphate Dehydrogenase

Abstract

Abstract When presented with glucose, an Escherichia coli mutant lacking both phosphoglucose isomerase and glucose 6-phosphate dehydrogenase accumulated, internally, high concentrations (approximately 50 mm) of glucose 6-phosphate. It also accumulated glucose in polysaccharide form. Other metabolism of glucose, if any, was very restricted. The accumulation of glucose 6-phosphate was associated with inhibition of growth on those carbon sources the metabolism of which requires functional fructose disphosphatase activity; inhibition of this enzyme in vitro by high concentrations of glucose 6-phosphate was shown.