Publication | Closed Access
Carcinoma after malignant conversion in vitro of epithelial-like cells from rat liver following exposure to chemical carcinogens.
114
Citations
0
References
1973
Year
Summary Epithelial-like CellsPathologyCell CultureTumor BiologyMalignant ConversionTissue DevelopmentHepatobiliary TumorSurgical PathologyCancer Cell BiologyToxicologyHepatotoxicityRadiation OncologyCancer ResearchHealth SciencesOncogenic AgentLiver PhysiologyHistopathologyCell BiologyMalignant DiseaseRat LiverTumoral PathologyHepatologyChemical CarcinogensLiver CancerMedicine
Summary Epithelial-like cells from rat liver were exposed in cell culture to aflatoxin B 1 , dimethylnitrosamine, N -nitrosomethylurea, N -hydroxy- N -2-fluorenylacetamide, or 7,12-dimethylbenz(a)anthracene. Microscopic observation revealed serveral morphological changes in almost all of the treated sublines but revealed no uniform, characteristic alterations common to all of the cultures. The injection of 5 to 20 × 10 6 treated cells into newborn or X-irradiated syngeneic rats yielded tumors, usually after latent periods of 2 to 8 months or an average of 7.8 months. The tumors displayed epithelial aspects and were diagnosed as carcinomas.