Structure of the glycine-rich, arginine-rich histone of the Novikoff hepatoma.

Abstract

The glycine-rich, arginine-rich histone of Novikoff hepatoma was hydrolyzed with trypsin, chymotrypsin, and thermolysin. The peptides obtained were analyzed and compared to similar peptides from the calf thymus glycine-rich, arginine-rich histone (14). Thirteen tryptic peptides were analyzed, and three were sequenced. Six chymotryptic and seven thermolysin peptides were analyzed. On the basis of similarity of the peptides isolated from Novikoff hepatoma and calf thymus glycine-rich, arginine-rich histones, it seems evident that the two histone structures are essentially identical.