Quantitative Determination of Fumonisins B₁and B₂by High-Performance Liquid Chromatography with Fluorescence Detection

TLDR

The study developed a high‑performance liquid chromatography method for quantifying fumonisins B1 and B2 using pre‑column derivatization with o‑phthaldialdehyde and fluorescence detection. The method involves purifying sample extracts on strong anion‑exchange cartridges, then analyzing naturally contaminated corn, mixed horse feed, and fungal cultures by isocratic HPLC with fluorescence detection. Detection limits are ~50 ng g⁻¹ for FB1 and ~100 ng g⁻¹ for FB2, with recoveries of 99.5 % and 85.9 % and high reproducibility.

Abstract

Abstract A high-performance liquid chromatographic method has been developed for the quantitative determination of the recently described mycotoxins, fumonisins B1 (FB1) and B2 (FB2) utilizing pre-column derivatization with o-phthaldialdehyde, isocratic elution, and fluorescence detection. Prior to analysis, sample extracts were purified on strong anion exchange cartridges. The method has been applied to the analysis of naturally contaminated corn and mixed horse feed samples as well as fungal culture material, for the presence of the mycotoxins. Detection limits are approximately 50 ng g−1 for FB1 and 100 ng g−1 for FB2. The method proved to be highly reproducible and recoveries of the toxins from the purification steps were found to be 99.5% and 85.9% for FB1 and FB2, respectively.