Recent Developments in Techniques for Terminal and Sequence Studies in Peptides and Proteins

Abstract

This chapter contains sections titled: Part I. The Fluorodinitrobenzene Method for Amino End Group Analysis. By A. L. Levy Introduction General Discussion of the Method Experimental Section Summary and Conclusions Part II. The Phenylisothiocyanate Method for Stepwise Degradation from the Amino End. By H. Frankel-Conrat Peptides Proteins Applications and Limitations III. The Carboxypeptidase Method for C-Terminal Group Analysis and Stepwise Degradation from the Carboxyl End. By J. Ieuan Harris Introduction Theoretical Considerations Development of the Method Purity of Substrate and Enzyme Experimental Procedures Biological Activity of Proteins after Removal of C-Terminal Groups Conclusion Part IV. General Considerations. By H. Franekel-Conrat, J. Ieuan Harris, and A. L. Levy