Fabrication of biocompatible lab‐on‐chip devices for biomedical applications by means of a 3D‐printing process

Abstract

A new microfluidic assembly method for semiconductor‐based biosensors using 3D‐printing technologies was proposed for a rapid and cost‐efficient design of new sensor systems. The microfluidic unit is designed and printed by a 3D‐printer in just a few hours and assembled on a light‐addressable potentiometric sensor (LAPS) chip using a photo resin. The cell growth curves obtained from culturing cells within microfluidics‐based LAPS systems were compared with cell growth curves in cell culture flasks to examine biocompatibility of the 3D‐printed chips. Furthermore, an optimal cell culturing within microfluidics‐based LAPS chips was achieved by adjusting the fetal calf serum concentrations of the cell culture medium, an important factor for the cell proliferation.