Regulation of protein kinase C by ionizing radiation.

Abstract

Members of the protein kinase C (PKC) gene family have been shown to play an important role in tumor promotion and regulation of cell growth. Experiments were designed to examine the effects of different qualities of ionizing radiation administered at a variety of doses and dose rates on the expression of PKC-specific mRNA in confluent Syrian hamster embryo cells. The results of these experiments showed that low-linear energy of transfer (LET) radiations (such as X-rays and gamma-rays) can induce increased expression of PKC mRNA within 1 h after radiation exposure. Levels of expression of PKC mRNA were increased 4- to 6-fold over unirradiated controls. Dose effects were evident, with increased accumulation of PKC mRNA at higher doses (ranging from 6 to 200 cGy). Induction of PKC mRNA occurred at a time when total cellular transcription was reduced following irradiation. Similar exposure of the cells to fission spectrum JANUS neutrons, however, had little effect on PKC mRNA expression. Modest induction (2-fold compared to untreated cells) occurred when irradiations were at very low dose rates (0.5 cGy/min). These results suggest that induction of PKC mRNA may be a step in the transformation process caused by ionizing radiation. In addition, they demonstrate that different qualities of radiation may regulate PKC differently.