Publication | Closed Access
Enhanced metabolism and mutagenesis of nitrosopyrrolidine in liver fractions isolated from chronic ethanol-consuming hamsters.
55
Citations
0
References
1979
Year
Enhanced MetabolismIsolated Liver FractionsOxidative StressChronic Ethanol ConsumptionToxicologyHepatotoxicityAlcohol DehydrogenasesHealth SciencesAniline HydroxylaseAldehyde DehydrogenaseBiochemistryLiver PhysiologyAlcohol-related Liver DiseaseMetabolomicsExperimental ToxicologyPharmacologyDrug-induced Liver InjuryChronic Ethanol-consuming HamstersLiverPhysiologyLiver FractionsMetabolismMedicineNitrosative StressCarbonyl Metabolism
The effect of chronic ethanol consumption on the ability of isolated liver fractions to metabolize the carcinogen N-nitrosopyrrolidine (NPY) was examined. Microsomal fractions of treated animals exhibited increased rates of alpha-hydroxylation of NPY. Similar increases in the specific activities of aniline hydroxylase, reduced nicotinamide adenine dinucleotide phosphate cytochrome c reductase, and the specific content of cytochrome P-450 were also observed. In contrast, no differences in the specific activities of benzo(a)pyrene hydroxylase or glucose-6-phosphatase were observed. Liver postmitochondrial supernatants from ethanol-consuming animals were able to produce 5 times more mutants than did control preparations. It is concluded that alpha-hydroxylation of NPY is probably the mechanism by which NPY is converted to a mutagen and that this pathway can be induced by ethanol.